rabbit polyclonal anti-phospho-smad1/5; dilution  (Cell Signaling Technology Inc)

Journal: Scientific Reports

Article Title: BMP2 expression in oral squamous cell carcinoma and its effects on SCC9 cell biological behavior

doi: 10.1038/s41598-025-96274-2

Figure Lengend Snippet: Protein expression profiles were analyzed through immunoblotting across experimental groups. Notably, GAPDH, Smad1/5, and p38 protein levels remained unaltered regardless of BMP2 expression status in SCC9 cells. BMP2 overexpression induced significant phosphorylation enhancement of Smad1/5 (52% increase, p = 0.017) and p38 (33% increase, p = 0.020). Conversely, BMP2 knockdown substantially attenuated Smad1/5 phosphorylation (76% reduction, p = 0.009) and p38 phosphorylation (34% reduction, p = 0.045), with asterisks denoting statistical significance. * p < 0.05, ** p < 0.01.

Article Snippet: Experiments in this study were conducted with the following components: fetal bovine serum (Life-ilab, China); Cell Counting Kit-8 (CCK8) kit (ZomanBio, China); Transwell chambers (Corning, USA); Radio Immunoprecipitation Assay Lysis buffer, anti-mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody, and anti-rabbit Smad1/5 polyclonal antibody (Boster Bio, China); anti-rabbit p-Smad1/5 (Bioss, China); anti-rabbit BMP2 polyclonal antibody, anti-rabbit p38 polyclonal antibody, and anti-rabbit p-p38 polyclonal antibody (Wanlei Biology, Liaoning); BMP2 small interfering RNA (si-BMP2), control small interfering RNA (si-NC), BMP2 -OE plasmid, control plasmid, and Transfect-Mate transfection reagent (GenePharma, China); and Quantitative Rea-ltime-PCR (qPCR) primers (Thermo Fisher, USA).

Techniques: Expressing, Western Blot, Over Expression, Knockdown

Journal: Scientific Reports

Article Title: BMP2 expression in oral squamous cell carcinoma and its effects on SCC9 cell biological behavior

doi: 10.1038/s41598-025-96274-2

Figure Lengend Snippet: The mRNA expression levels of Smad1/4/5 and p38 in SCC9 cells were quantitatively analyzed using qPCR under different BMP2 modulation conditions. ( A ) Following BMP2 knockdown, significant upregulation of Smad1, Smad4, Smad5, and p38 mRNA expression was observed, with respective increases of 110%, 61%, 36%, and 38% compared to control groups ( p < 0.05 for all comparisons). ( B ) Conversely, BMP2 overexpression resulted in marked downregulation of these targets, showing respective reductions of 25%, 29%, 16%, and 22% in mRNA expression levels ( p < 0.05 for all measurements). All experimental data demonstrated statistically significant differences between treatment groups and corresponding controls. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Experiments in this study were conducted with the following components: fetal bovine serum (Life-ilab, China); Cell Counting Kit-8 (CCK8) kit (ZomanBio, China); Transwell chambers (Corning, USA); Radio Immunoprecipitation Assay Lysis buffer, anti-mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody, and anti-rabbit Smad1/5 polyclonal antibody (Boster Bio, China); anti-rabbit p-Smad1/5 (Bioss, China); anti-rabbit BMP2 polyclonal antibody, anti-rabbit p38 polyclonal antibody, and anti-rabbit p-p38 polyclonal antibody (Wanlei Biology, Liaoning); BMP2 small interfering RNA (si-BMP2), control small interfering RNA (si-NC), BMP2 -OE plasmid, control plasmid, and Transfect-Mate transfection reagent (GenePharma, China); and Quantitative Rea-ltime-PCR (qPCR) primers (Thermo Fisher, USA).

Techniques: Expressing, Knockdown, Control, Over Expression